Replication and packaging of helper-dependent adenoviral vectors
نویسندگان
چکیده
منابع مشابه
Feasibility of Applying Helper-Dependent Adenoviral Vectors for Cancer Immunotherapy
Adenoviruses (Ads) infect a broad range of tissue types, and derived vectors have been extensively used for gene therapy. Helper-dependent Ad vectors (HDAds), devoid of viral coding sequences, allow for insertion of large or multiple transgenes in a single vector and have been preclinically used for the study of genetic disorders. However, the clinical application of Ad vectors including HDAds ...
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In the majority of potential applications gene therapy will require an effective transfer of a transgene in vivo resulting in high-level and long-term transgene expression, all in the absence of significant toxicity or inflammatory responses. The most efficient vehicles for delivery of foreign genes to the target tissues are modified adenoviruses. Adenoviral vectors of the first generation, des...
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Adenoviral (Ad)-mediated in vivo gene transfer and expression is limited in part by cellular immune responses to viralencoded proteins. In an attempt to diminish these responses, we have previously developed and described helperdependent (HD) Ad vectors in which the viral protein coding sequences are completely deleted. These vectors provided efficient delivery, and greater safety which represe...
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Adenoviruses (Ads) have shown great utility as vectors for the delivery of genes to mammalian cells, partly because of their ability to infect a wide range of different cell types independent of the replicative state of the cell. However, Ads do not transduce mature muscle efficiently because of low levels of the natural viral primary receptor, the coxsackie virus and adenovirus receptor, on th...
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For gene therapy of inherited diseases, targeted integration/gene repair through homologous recombination (HR) between exogenous and chromosomal DNA would be an ideal strategy to avoid potentially serious problems of random integration such as cellular transformation and gene silencing. Efficient sequence-specific modification of chromosomes by HR would also advance both biological studies and ...
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ژورنال
عنوان ژورنال: Gene Therapy
سال: 2002
ISSN: 0969-7128,1476-5462
DOI: 10.1038/sj.gt.3301668